Biofiltration is an ecological engineering solution that uses natural biological processes to clean contaminated air or water. This method involves channeling a polluted stream, whether gaseous or liquid, through a specialized filter material where living organisms reside. The system’s primary function is to sustainably remove pollutants by utilizing the metabolic capabilities of these resident microbes. Biofiltration is an adaptable, cost-effective, and environmentally conscious purification method used across various sectors.
Core Principles of Biofiltration
The mechanism of biofiltration relies on a two-step process: sorption and subsequent biodegradation of contaminants. Pollutants first contact the filter material and the attached microbial community, where they are physically trapped or adsorbed onto the surfaces. This initial capture concentrates the contaminants, making them available for breakdown by the organisms.
The active component is the biofilm, a thin layer of immobilized microorganisms such as bacteria, fungi, and protozoa, which colonizes the filter media surface. These microbes consume the trapped organic and inorganic pollutants, transforming them into harmless end products like carbon dioxide, water, and mineral salts. The continuous flow of contaminated fluid provides the necessary food source and moisture for the biofilm to thrive.
In water treatment, biofilters often target nitrogen compounds through specific microbial pathways. Nitrification is an aerobic, two-step process where autotrophic bacteria first oxidize toxic ammonia into nitrite, which is then converted into nitrate. This conversion is performed sequentially by different groups of slow-growing bacteria, such as Nitrosomonas and Nitrospira.
For complete nitrogen removal, denitrification is employed, converting nitrate into inert nitrogen gas that vents out of the system. This process is anaerobic, occurring in filter areas where oxygen levels are extremely low. Air purification systems rely on the broad oxidation of volatile organic compounds (VOCs) and odorous gases, converting them directly into carbon dioxide and water within the biofilm.
Essential Components of a Biofilter
A functional biofiltration system requires three main physical elements: the filter media, a containment structure, and a delivery mechanism. The filter media is the physical substrate that provides a massive surface area for microbial communities to attach and grow. Media materials are selected based on the application and often include organic materials like compost and wood chips, or inorganic substrates such as sand, gravel, activated carbon, or specialized synthetic plastic shapes.
The choice of media influences the flow rate, oxygen availability, and the water-holding capacity necessary to keep the biofilm moist. For instance, high-porosity media is needed in air biofilters to minimize the energy required to push air through the bed. In water systems, media with a high surface-to-volume ratio, such as plastic biomedia, is preferred to maximize the concentration of active biomass within a small reactor space.
The containment structure houses the filter media, whether it is an engineered basin, a filter bed, or a closed reactor tank. This structure ensures the contaminated fluid passes entirely through the active media layer before being discharged as treated effluent.
The delivery system introduces and distributes the polluted air or water evenly across the media surface. In air systems, this often involves a fan and an air plenum—a chamber beneath the media that diffuses the gas flow—to prevent channeling and ensure uniform contact time. For water systems, nozzle systems, diffusers, or strategically placed pipes are used to spray or percolate the water, ensuring the entire volume interacts with the active biofilm.
Major Applications of Biofiltration
Biofiltration is widely used in urban environments for stormwater management to mitigate the impact of runoff from paved surfaces. Systems like bioswales and rain gardens are engineered soil and plant-based filters that capture stormwater, reducing peak flow and removing pollutants. These filters effectively remove sediments, heavy metals, and hydrocarbons through physical straining, soil adsorption, and microbial degradation.
In the agricultural and industrial sectors, biofilters are used for air purification to control odors and remove volatile organic compounds (VOCs). These systems treat exhaust air from sources like livestock facilities or manufacturing plants by passing the air through a moist media bed. This process achieves high removal efficiencies for compounds like hydrogen sulfide and ammonia, significantly reducing odor nuisance in surrounding communities.
Wastewater treatment plants utilize biofiltration, often as a tertiary polishing step, to remove residual contaminants before discharge. These biofilters reduce the concentration of organic matter and nitrogen compounds remaining after primary and secondary treatment. The compact nature of some biofilter designs makes them advantageous in locations where space is limited, such as densely populated urban areas.
A specialized application of biofiltration is in Recirculating Aquaculture Systems (RAS), where it maintains water quality for fish. Fish excrete toxic ammonia as a waste product, and the biofilter’s primary purpose is to rapidly convert this ammonia into nitrate through the nitrification process. This biological conversion allows the system to reuse the water continuously, minimizing the need for constant, large-volume water exchange.

