Head lice infestation begins with tiny eggs cemented to the hair shaft, known as nits. Nits represent the first stage in the life cycle of the parasitic insect, Pediculus humanus capitis. Understanding the timeline for these nits to hatch is necessary because it dictates the most effective strategy for complete removal. Successful treatment relies on breaking the reproductive cycle.
The Incubation Period
The incubation period for a nit is typically 6 to 9 days after it has been laid by the adult female louse. This relatively short period is a direct function of the environment required for the egg’s development.
The female louse carefully glues the nit to the hair shaft very close to the scalp, usually within a quarter of an inch (about 6 millimeters). This precise placement ensures the egg receives the warmth and humidity necessary for it to develop and hatch. Nits found further than 1/4 inch from the scalp are generally non-viable, as they are too far from the heat source of the scalp to survive.
The Nymph Stage and Maturation
Once the nit hatches, a small, immature louse emerges, known as a nymph. The nymph looks like a miniature version of the adult louse but is smaller, about the size of a pinhead. To survive and grow, the nymph must immediately begin feeding on human blood, staying close to the scalp.
The nymph stage is a period of rapid growth and molting, where the insect sheds its outer layer three times. The timeline from a newly hatched nymph to a reproductive adult louse is approximately 7 to 12 days. Once the nymph completes its third molt, the female louse is mature and can begin laying eggs within a day or two of mating.
Why Timing Treatment is Critical
Understanding the biological timelines of the nit and the nymph is the foundation for successful treatment. Most treatments are highly effective at killing live, crawling lice and nymphs, but they are often less effective at penetrating and killing the nits. The hard shell of the nit protects the developing embryo from the chemical agents in the initial application.
A single treatment is rarely sufficient because the surviving nits will continue to hatch over the following days, starting a new generation. The goal of the second treatment is to kill all the lice that have hatched since the first application, but before they have time to mature and lay new eggs. Since nits hatch between days 6 and 9, and nymphs take about 7 to 12 days to mature, a second treatment is usually scheduled between days 7 and 10.
This specific timing ensures that all lice have emerged from the protective nit stage and are vulnerable to the treatment. By treating again on or around day 9, all newly hatched nymphs are killed before any of the females can reach sexual maturity and start laying their own eggs, effectively breaking the reproductive cycle. A third application may sometimes be recommended around day 14.

