Osmosis is a passive process in biology that governs the movement of water. It is defined as the net movement of water molecules across a selectively permeable membrane. This movement is spontaneous and does not require the cell to expend energy, classifying it as passive transport. Water transport is often facilitated by specialized protein channels called aquaporins embedded within cell membranes.
How Osmosis Works: The Movement of Water
The mechanism of osmosis is driven by an imbalance in the concentration of dissolved substances, known as solutes, on either side of a semipermeable membrane. This membrane allows water (the solvent) to pass through but blocks larger solute particles. The presence of a solute effectively lowers the concentration of free water molecules in that area.
Water molecules move down their concentration gradient, traveling from a region where water is highly concentrated to a region where it is less concentrated. This is equivalent to water moving from a solution with a low solute concentration to one with a high solute concentration. The movement continues until the solute concentration is equalized on both sides, or until hydrostatic pressure opposes further net flow.
The difference in water concentration creates a force called osmotic pressure, which is the pressure required to stop the net movement of water across the membrane.
Understanding Tonicity and Cellular Environments
Tonicity describes the concentration of solutes in the solution surrounding a cell, determining the direction and extent of water movement. In an isotonic solution, the solute concentration outside the cell equals the concentration inside the cell. Water flows equally in and out, resulting in no net change in cell volume.
In a hypertonic environment, the external solution has a higher solute concentration than the cell’s interior. Water moves out of the cell toward the higher solute concentration, causing the cell to shrink. For human red blood cells, this shrinking process is called crenation.
Conversely, a hypotonic solution has a lower solute concentration than the cell’s cytoplasm, causing a net influx of water into the cell and leading to swelling.
If an animal cell takes in too much water, the internal pressure can cause the cell to burst, a process known as lysis or hemolysis.
Essential Roles in Human Biology and Plant Life
Osmosis is central to maintaining stable internal conditions, or homeostasis, in complex organisms. In the human body, a major application is the reabsorption of water within the kidneys. As blood is filtered in the kidney’s nephrons, the surrounding tissue fluid is made increasingly concentrated by actively transporting solutes out of the filtrate.
This high solute concentration creates an osmotic gradient that draws water out of the nephron tubules and back into the bloodstream. Water reabsorption is regulated by hormones, such as vasopressin (antidiuretic hormone), which controls the permeability of the tubule walls to water. Osmosis also facilitates the absorption of water from the digestive tract into the bloodstream, following the movement of absorbed nutrients and electrolytes.
In plant life, osmosis is the primary mechanism by which water is absorbed from the soil through the root hairs. The cytoplasm and vacuole of a root cell typically have a higher concentration of solutes than the soil water, creating a gradient that drives water inward.
This continuous water uptake generates turgor pressure, the internal hydrostatic pressure that pushes the cell membrane against the cell wall. Maintaining adequate turgor pressure provides structural rigidity to plant stems and leaves, allowing the plant to remain upright. If a plant is not watered, the soil solution can become hypertonic to the root cells, causing water to flow out and the cell to lose turgor, resulting in wilting.

