How to Put On an Isolation Gown and Remove It Safely

Putting on an isolation gown correctly takes about 30 seconds, but each step matters. The gown needs to fully cover your torso from neck to knees, your arms to the wrists, and wrap completely around your back with no gaps. Here’s exactly how to do it, plus how it fits into the full sequence of protective equipment.

Step-by-Step Donning Instructions

Before you touch the gown, perform hand hygiene. Then follow these steps in order:

  • Pick up the gown and unfold it. Hold it by the shoulder seams and let it fall open in front of you. The opening goes in the back, so the inside of the gown faces your body.
  • Slip your arms through the sleeves. Slide both arms into the sleeves one at a time, pushing your hands through the cuffs until they emerge at the wrists. Don’t bunch the sleeves up your forearms.
  • Secure the neck tie. Reach behind your head and tie or fasten the closure at the back of your neck. This anchors the gown in place and keeps the neckline from sagging.
  • Wrap the gown around your back. Pull the edges of the gown so they overlap across your back, leaving no exposed clothing or skin behind you.
  • Secure the waist tie. Tie the waist fasteners behind you. This keeps the gown snug and prevents it from shifting or flapping open during movement.

The gown goes on first in the PPE sequence. After the gown, you put on your mask or respirator, then eye protection, and finally gloves. This order ensures that each piece overlaps correctly with the next.

Getting the Glove-to-Cuff Overlap Right

One of the most important details is where the gown cuffs meet your gloves. Your gloves should extend over and cover the wrists of the gown, creating a continuous barrier with no exposed skin between the cuff and the glove. If you’re wearing two layers of gloves (common in higher-risk settings), the inner glove cuffs go under the gown sleeve, and the outer gloves then extend over the gown cuffs.

If your gloves are too small to overlap the cuffs, size up. A gap between glove and gown is one of the easiest ways for fluids or pathogens to reach your skin.

Choosing the Right Gown Level

Isolation gowns are rated from Level 1 to Level 4 based on how well they resist fluid penetration. Using the wrong level for the task can leave you underprotected.

  • Level 1 (minimal risk): Basic patient care, standard isolation rooms, visitor cover gowns. These resist a light water spray but not much else.
  • Level 2 (low risk): Blood draws, suturing, ICU care, pathology lab work. These handle water spray and offer some resistance to fluid under pressure.
  • Level 3 (moderate risk): Arterial blood draws, IV insertion, emergency room and trauma situations. Stronger fluid resistance under sustained contact.
  • Level 4 (high risk): Long, fluid-intensive procedures, surgery, or suspected infectious diseases. These are the only gowns tested and rated for resistance to blood and viral penetration.

If you’re dealing with a patient who has a suspected non-airborne infectious disease, a Level 4 gown is the appropriate choice. Levels 1 through 3 provide increasing splash protection but are not impermeable to viruses.

Non-Sterile vs. Sterile Gowns

Most isolation gowns used for standard patient care are non-sterile. These are classified by the FDA as Class I devices, meaning they go through less regulatory review. They’re designed for low or minimal risk situations where you’re protecting yourself from body fluids during routine care.

Sterile gowns are required for surgical procedures. Surgical isolation gowns, which offer larger protected zones than standard surgical gowns, are Class II medical devices and go through a more rigorous premarket review. The donning technique for sterile gowns is more controlled to avoid contaminating the outer surface, typically involving an assisted gowning technique where another person helps secure the back ties without touching the sterile front.

For the standard non-sterile isolation gown most people are searching about, you can tie your own back closures without concern about maintaining a sterile field.

How to Safely Remove the Gown

Taking the gown off is actually the higher-risk step. The outside of the gown is now contaminated, so the goal is to remove it without letting that outer surface touch your skin, hair, or clothing.

Start by untying the waist tie, then the neck tie. Grasp the gown at the shoulders from behind and pull it away from your body, peeling it from back to front. As it comes off, fold or roll it so the contaminated outside surface rolls inward on itself. Dispose of it immediately in the appropriate waste container, then perform hand hygiene right away.

In the full PPE removal sequence, gloves come off first, followed by hand hygiene, then the gown, then eye protection, then the mask or respirator, with hand hygiene again at the end.

Common Mistakes to Avoid

The most frequent errors happen not during donning but during removal, especially after long shifts. Fatigue causes people to rush through the doffing sequence, skip steps, or accidentally touch the contaminated outer surface of the gown. Research on healthcare workers during COVID-19 found that extended hours of intensive work made staff significantly more prone to mistakes during removal, increasing their risk of infection.

During donning, the most common problems are:

  • Leaving the back uncovered. If you don’t wrap the gown fully around your back and secure the waist tie, your scrubs or clothing are exposed.
  • Gaps at the wrists. Gloves that don’t overlap the gown cuffs leave a strip of bare skin vulnerable to fluid contact.
  • Wrong donning order. Putting gloves on before the gown, or the gown on after the mask, breaks the overlap system that keeps each layer sealed against the next.
  • Using the wrong gown level. A Level 1 gown in a moderate-risk situation offers false confidence. Match the gown to the exposure risk.

If your facility has a buddy system or a mirror near the donning station, use them. A second set of eyes catches gaps you can’t see on your own back, and a quick visual check before entering the room takes only a few seconds.