Capillary puncture, often recognized as a fingerstick or heelstick, is a minimally invasive technique used to collect a small blood sample for testing. This method uses a specialized lancet to prick the skin, accessing the tiny blood vessels just beneath the surface. The procedure is widely used in medical diagnostics, especially when only a small volume of blood is required or when traditional venipuncture is difficult. It is a fundamental approach for quick, point-of-care testing in various healthcare settings, including hospitals, clinics, and at home.
Defining Capillary Puncture
Capillary puncture specifically targets the capillaries, which are the smallest blood vessels and serve as the connection points between the arterial and venous systems. These vessels are where the exchange of oxygen, carbon dioxide, nutrients, and waste products occurs. Because of this unique location, the blood collected from a capillary puncture is not purely venous or arterial blood.
The sample is actually a mixture of blood from arterioles, venules, and the capillaries themselves, along with interstitial and intracellular fluids from surrounding tissues. This composition means that capillary blood values may slightly differ from venous blood for certain components, such as higher hemoglobin and hematocrit, but lower levels of potassium and calcium. Clinicians account for this factor when interpreting results. This collection method is preferred over a standard vein draw because it is less painful, requires a much smaller volume of blood, and is the only feasible option for infants or patients with fragile veins.
The Capillary Puncture Procedure
The process begins with careful site selection, which is determined by the patient’s age. For adults and older children, the preferred site is the ring or middle finger, specifically the fleshy pad slightly off the center. For infants younger than six months, a heelstick is performed on the most medial or lateral portion of the plantar surface of the heel, taking care to avoid the bone.
Before the puncture, the chosen site is warmed for three to five minutes to increase blood flow and ensure a sufficient sample. The area is then cleaned with an antiseptic, like 70% isopropyl alcohol, which must be allowed to air dry completely before the lancet is used. A sterile, single-use, safety-engineered lancet is used to make a quick, deliberate puncture.
The first drop of blood that appears must be wiped away with a clean piece of gauze because it may contain excess tissue fluid or antiseptic residue that could contaminate the sample. Subsequent blood drops are collected into a microcollection tube or onto a test strip by gently touching the collection device to the drop. Avoid “milking” or excessively squeezing the puncture site, as this can force tissue fluid into the sample, diluting the blood and leading to inaccurate results.
Common Applications for Capillary Blood
The small sample size and ease of collection make capillary puncture suitable for a range of diagnostic tests. A primary use is for glucose monitoring, allowing individuals with diabetes to check their blood sugar levels multiple times a day at home or in a clinical setting. This point-of-care testing provides immediate results that inform daily management decisions.
Capillary blood is the standard sample source for newborn screening programs, where a few drops collected via a heelstick are placed on filter paper. These tests screen for dozens of congenital and metabolic disorders shortly after birth, making early detection and intervention possible. This method is routinely employed for quick checks of hemoglobin and hematocrit levels, which help screen for conditions like anemia. Rapid diagnostic tests for certain infectious diseases or coagulation checks frequently rely on a capillary sample when a small volume and fast turnaround time are necessary.
Preparation and Post-Procedure Care
Proper preparation significantly improves the success rate and patient comfort of a capillary puncture. Hydration is beneficial, as it helps maintain good blood volume, and the patient should ensure the limb to be sampled is warm. Warming the finger or heel, either with a warm compress or by washing with warm water, encourages blood flow to the puncture site, helping a sufficient blood drop form quickly. The patient should position the hand or foot lower than the heart during the collection to assist with gravity-fed blood flow.
Once the sample has been collected, the patient should apply firm pressure to the puncture site with a clean gauze pad to stop any bleeding. A small bandage or adhesive strip can then be applied to protect the site. Patients should monitor the area for any signs of prolonged bleeding, excessive bruising, or infection in the days following the procedure. Infants under the age of two should not have adhesive bandages applied due to the risk of skin irritation or being a choking hazard.

