Protein is one of the three primary macronutrients, alongside fats and carbohydrates. It is a large, complex substance present in every cell and represents about 20% of the body’s total weight. Unlike carbohydrates and fats, which are primarily used for energy, protein is mainly dedicated to structure and function. Because this macronutrient is constantly being broken down and rebuilt, consistent replenishment through diet is necessary for maintaining health.
The Building Blocks of Protein
The fundamental components of protein are small organic compounds known as amino acids. Approximately 20 different types of amino acids serve as the basic units for all proteins in the human body. These amino acids link together, forming long chains called polypeptides. The specific order of amino acids in the polypeptide chain determines how the protein folds into its unique three-dimensional structure, which dictates its specific function.
Amino acids are categorized based on how the body obtains them. Eleven are non-essential because the body can synthesize them internally. The remaining nine are classified as essential because the body cannot manufacture them and must obtain them through the diet. These nine essential amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine) are necessary for building and repairing tissues. If even one is missing, the body’s ability to create necessary proteins becomes limited.
Essential Roles in the Human Body
The functions of protein extend far beyond supporting muscle tissue, encompassing a vast array of biological processes.
Proteins act as enzymes, which are biological catalysts that speed up chemical reactions within cells. Enzymes are responsible for processes ranging from breaking down food during digestion to facilitating metabolic pathways that produce energy. For example, the enzyme lactase breaks down the sugar lactose.
Proteins also provide structural integrity to the body’s tissues. Collagen is the most abundant protein, providing strength and elasticity to skin, bones, tendons, and cartilage. Keratin is another structural protein that forms the primary component of hair and nails.
Proteins are dedicated to the transportation and storage of various substances. Hemoglobin, found in red blood cells, binds to oxygen in the lungs and delivers it to tissues. Other transporter proteins embedded in cell membranes regulate which nutrients, ions, and waste products enter or exit the cell.
Proteins also play a part in the immune and endocrine systems. Antibodies recognize and neutralize foreign invaders like bacteria and viruses. Certain hormones, such as insulin and human growth hormone, are proteins that function as chemical messengers regulating processes like blood sugar levels and body development.
Dietary Sources and Quality
Obtaining protein requires understanding both the quantity and quality of the food, which is determined by its amino acid profile. Proteins are categorized as either complete or incomplete based on whether they contain all nine essential amino acids. Complete proteins provide all essential amino acids in sufficient quantities.
Animal-based foods are common examples:
- Meat
- Poultry
- Fish
- Eggs
- Dairy products
Some plant sources, such as soy, quinoa, and amaranth, also contain all nine essential amino acids.
In contrast, incomplete proteins are low in one or more essential amino acids. Most plant-based sources fall into this category:
- Legumes
- Nuts
- Seeds
- Grains
The limiting amino acid prevents the body from optimally utilizing the protein source by itself. Individuals following a plant-based diet can meet their needs by combining different incomplete sources to form a complete profile. This is known as consuming complementary proteins. A classic example is combining grains (low in lysine) with legumes (low in methionine), such as rice and beans. Complementary proteins do not need to be consumed in the same meal, but rather over the course of the day.
Calculating Daily Protein Needs
Determining daily protein needs varies based on several personal factors. For a healthy, sedentary adult, the Recommended Dietary Allowance (RDA) is 0.8 grams of protein per kilogram of body weight. For example, a person weighing 70 kilograms (154 pounds) would need roughly 56 grams per day. This baseline increases significantly for those with higher demands. Active individuals and athletes require greater intake to support muscle repair and recovery.
Protein Needs by Activity Level
For athletes, recommendations range from 1.2 to 2.0 grams per kilogram of body weight, depending on training intensity. Older adults (over 65) also have increased needs, often requiring 1.0 to 1.2 grams per kilogram. This higher amount helps counteract age-related muscle loss (sarcopenia). Spreading protein intake evenly across all meals is recommended to optimize the body’s utilization of amino acids. Individuals with underlying health conditions, particularly kidney issues, should consult a healthcare provider before significantly increasing their protein consumption.

