The vagina is a highly adaptable, fibromuscular canal that forms a part of the internal female reproductive tract. This structure extends from the external opening, located in the vulva, up to the cervix at the base of the uterus. Its structure enables its roles in passage, protection, lubrication, and expansion.
Gross Anatomy and Positioning
The vagina is positioned within the lower pelvis, situated between several other internal organs. In an unaroused state, this collapsed tube typically measures between 7.5 and 10 centimeters in length. The canal is not oriented vertically but rather slopes upward and backward from the external opening, forming an angle with the uterus.
Its anatomical placement is close to the urinary and digestive tracts. It lies directly anterior to the rectum and the anal canal, and posterior to the urinary bladder and the urethra.
The upper boundary of the canal connects to the cervix. This connection forms recesses called the fornices. At the lower end, the canal opens to the outside at the vaginal orifice, which is often partially covered by a thin fold of tissue known as the hymen. The entire structure is supported and kept in place by various ligaments and the muscles of the pelvic floor.
Tissue Composition and Flexibility
The vaginal wall is constructed of three distinct layers that provide strength and elasticity. The outermost layer is the adventitia, a sheath of dense connective tissue rich in collagen and elastic fibers that anchors the canal to surrounding tissues. The middle layer is the muscularis, composed primarily of smooth muscle fibers arranged in inner circular and outer longitudinal layers.
The innermost layer is the mucosa, which is lined by non-keratinized stratified squamous epithelium. The lining lacks mucous-secreting glands. Instead, moisture and lubrication are provided by a process called transudation, where fluid seeps through the porous walls of the tissue, supplemented by mucus from the cervix.
A defining feature of the inner wall is the presence of transverse folds known as rugae. These ridges are composed of the mucosal lining and underlying connective tissue, allowing the walls to maintain a wrinkled appearance when relaxed. The rugae are instrumental in the organ’s elasticity, enabling significant expansion. They also increase the surface area of the lining, which aids in maintaining natural moisture and housing the vaginal flora.
Functional Roles of the Structure
The elasticity provided by the rugae and the robust connective tissue of the wall permits the canal to accommodate a penis during sexual intercourse. The canal also serves as the initial pathway for sperm to travel toward the cervix and uterus, facilitating conception.
The structure functions as the exit route for menstrual fluid. The lower third of the canal contains nerve endings, which contribute to the sensation experienced during sexual activity.
The structure’s most demanding function is facilitating childbirth, where the vagina acts as the final portion of the birth canal. The flexibility allows the canal to dilate dramatically. Following delivery, the structural components are designed to recoil, allowing the tissue to return substantially toward its pre-pregnancy state.
Structural Changes Over the Lifespan
The physical architecture of the vagina is highly responsive to the fluctuating levels of sex hormones. During the reproductive years, high estrogen levels cause the wall to be thick, moist, and highly elastic. Estrogen stimulation encourages the surface cells to store glycogen, which is then metabolized by resident bacteria to create an acidic environment.
Before puberty and after menopause, structural characteristics change significantly due to low estrogen exposure. The decrease in estrogen leads to a condition where the vaginal walls become thinner, drier, and less elastic. This atrophy can cause the tissues to be more susceptible to irritation and injury.
The rugae may also become less prominent and flatten out over the course of a lifetime, particularly following childbirth or with age-related atrophy. This reduction in the folds contributes to decreased elasticity and a narrower canal.

